Anticorps Polyclonal de lapin anti-EYA1

• Phare
• Validé par KD/KO

EYA1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 22658-1-AP

Synonymes

BOP, BOR, EYA1, Eyes absent homolog 1

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Galerie de données de validation

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  WB analysis using 22658-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22658-1-AP (EYA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 22658-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22658-1-AP (EYA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 22658-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 22658-1-AP (EYA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22658-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22658-1-AP (EYA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 22658-1-AP

  IP result of anti-EYA1(IP:22658-1-AP, 4ug; Detection:22658-1-AP 1:4000) with HepG2 cells lysate 2600 ug.

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  IHC staining of human kidney using 22658-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22658-1-AP (EYA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 22658-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22658-1-AP (EYA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 22658-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EYA1 antibody (22658-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, cellules HuH-7, cellules NCCIT, tissu de côlon de souris, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

22658-1-AP cible EYA1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EYA1 Protéine recombinante Ag18489
• Nom complet: eyes absent homolog 1 (Drosophila)
• Masse moléculaire calculée: 592 aa, 65 kDa
• Poids moléculaire observé: 60 kDa, 50 kDa
• Numéro d’acquisition GenBank: BC121799
• Symbole du gène: EYA1
• Identification du gène (NCBI): 2138
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The EYA1 (Eyes absent homolog 1) gene is the human homologue of the Drosophila Eya1 gene, which is essential for eye development in that species. There are 4 different isoforms of EYA1 (EYA1A, EYA1B, EYA1C, EYA1D), which are composed of 559 amino acids (AA), 592 AA, 592 AA and 557 AA, respectively. It should be noted that isoforms B and C have the same length; however, they could be considered different variants because of change in amino acidic composition (PMID: 24803398).

Publications

Species	Application	Title
human	WB	Cell Rep; YTHDF1 phase separation triggers the fate transition of spermatogonial stem cells by activating the IκB-NF-κB-CCND1 axis; Authors - Qian Fang
human	IHC,IF	Front Oncol; SIX1 Activates STAT3 Signaling to Promote the Proliferation of Thyroid Carcinoma via EYA1.; Authors - Deguang Kong
human	WB,IHC	Oncotarget; Eyes absent gene (EYA1) is a pathogenic driver and a therapeutic target for melanoma.; Authors - Joshua Jiawei Zhou ; KD Validated
human	WB	Stem Cell Res Ther; Co-transplantation with adipose-derived cells to improve parathyroid transplantation in a mice model.; Authors - Qiuxia Cui
human	IHC	Clin Breast Cancer; Dachshund 1 is Differentially Expressed Between Male and Female Breast Cancer: A Matched Case-Control Study of Clinical Characteristics and Prognosis.; Authors - Qiuxia Cui
rat	WB,IP	J Mol Neurosci; MES23.5 DA Immortalized Neuroblastoma Cells Self-protect Against Early Injury by Overexpressing Glial Cell-derived Neurotrophic Factor via Akt1/Eya1/Six2 Signaling.; Authors - Jin Gao