Anticorps Polyclonal de lapin anti-EYA2

EYA2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11314-1-PBS

Synonymes

EC:3.1.3.48, EAB1

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Galerie de données de validation

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  WB analysis using 11314-1-AP (same clone as 11314-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11314-1-AP (EYA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11314-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11314-1-AP (same clone as 11314-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11314-1-AP (EYA2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11314-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11314-1-AP (same clone as 11314-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EYA2 antibody (11314-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11314-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11314-1-PBS cible EYA2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EYA2 Protéine recombinante Ag1860
• Nom complet: eyes absent homolog 2 (Drosophila)
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 56 kDa and 70 kDa
• Numéro d’acquisition GenBank: BC013882
• Symbole du gène: EYA2
• Identification du gène (NCBI): 2139
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EYA2, also named EAB1, belongs to the HAD-like hydrolase superfamily and EYA family. It coactivates SIX1. The Six1-EYA2 interaction may inhibit breast cancer progression. EYA2 is required for Six1-activated TGF-beta signaling.(PMID:21706047) It may be involved in the development of the eye. This protein has 3 isoforms produced by alternative splicing with the molecular weight of 59 kDa, 57 kDa, and 50 kDa. It can be detected two bands of 70 kDa and 56 kDa in the neuroblastoma cells by western blot(PMID:12039049).

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