Anticorps Polyclonal de lapin anti-EYA3

• Phare
• Validé par KD/KO

EYA3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 21196-1-AP

Synonymes

EC:3.1.3.48

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Galerie de données de validation

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  WB analysis of HeLa using 21196-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21196-1-AP (EYA3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 21196-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21196-1-AP (EYA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21196-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21196-1-AP (EYA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21196-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21196-1-AP (EYA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 21196-1-AP

  IP result of anti-EYA3 (IP:21196-1-AP, 4ug; Detection:21196-1-AP 1:1000) with L02 cells lysate 1800ug.

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  IHC staining of human breast cancer using 21196-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21196-1-AP (EYA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 21196-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21196-1-AP (EYA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 21196-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21196-1-AP (EYA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21196-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21196-1-AP (EYA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 21196-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EYA3 antibody (21196-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of A431 using 21196-1-AP

  1x10^6 A431 cells were intracellularly stained with 0.25 ug EYA3 Polyclonal antibody (21196-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules L02
• Résultats positifs en IP: cellules L02
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

21196-1-AP cible EYA3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EYA3 Protéine recombinante Ag15544
• Nom complet: eyes absent homolog 3 (Drosophila)
• Masse moléculaire calculée: 573 aa, 63 kDa
• Poids moléculaire observé: 63-66 kDa
• Numéro d’acquisition GenBank: BC029500
• Symbole du gène: EYA3
• Identification du gène (NCBI): 2140
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EYA3(Eyes absent homolog 3) belongs to the HAD-like hydrolase superfamily.Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis.EYA3, like EYA1, is expressed in the ninth week of human development and may also underlie developmental defects(PMID:9020840).It has 2 isoforms produced by alternative splicing.This antibody is specific to EYA3.

Publications

Species	Application	Title
human	WB,IHC,IF	Nat Commun; The EYA3 tyrosine phosphatase activity promotes pulmonary vascular remodeling in pulmonary arterial hypertension.; Authors - Yuhua Wang
		Sci Rep; WDR1 is a novel EYA3 substrate and its dephosphorylation induces modifications of the cellular actin cytoskeleton.; Authors - Mihaela Mentel
human	WB,IHC	Sci Rep; EYA3 promotes the tumorigenesis of gastric cancer through activation of the mTORC1 signaling pathway and inhibition of autophagy; Authors - Zhen Xu ; KD Validated
human	WB,IP	Int J Mol Sci; Analysis of EYA3 Phosphorylation by Src Kinase Identifies Residues Involved in Cell Proliferation.; Authors - Aura E Ionescu
human	WB	PLoS One; Structure-Activity Relationships of Benzbromarone Metabolites and Derivatives as EYA Inhibitory Anti-Angiogenic Agents.; Authors - Ram Naresh Pandey
	WB	J Biol Chem; Biochemical characterization of the Eya and PP2A-B55α interaction; Authors - Christopher Alderman

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