Anticorps Polyclonal de lapin anti-EYA3

• Phare
• Validé par KD/KO

EYA3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21196-1-PBS

Synonymes

EC:3.1.3.48

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Galerie de données de validation

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  WB analysis of HeLa using 21196-1-AP (same clone as 21196-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21196-1-AP (EYA3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 21196-1-AP (same clone as 21196-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 21196-1-AP (EYA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 21196-1-AP (same clone as 21196-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21196-1-AP (EYA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 21196-1-AP (same clone as 21196-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 21196-1-AP (EYA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

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  IP experiment of L02 using 21196-1-AP (same clone as 21196-1-PBS)

  IP result of anti-EYA3 (IP:21196-1-AP, 4ug; Detection:21196-1-AP 1:1000) with L02 cells lysate 1800ug. This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 21196-1-AP (same clone as 21196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21196-1-AP (EYA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 21196-1-AP (same clone as 21196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21196-1-AP (EYA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 21196-1-AP (same clone as 21196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21196-1-AP (EYA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 21196-1-AP (same clone as 21196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21196-1-AP (EYA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 21196-1-AP (same clone as 21196-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EYA3 antibody (21196-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 21196-1-AP (same clone as 21196-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug EYA3 Polyclonal antibody (21196-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21196-1-PBS cible EYA3 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EYA3 Protéine recombinante Ag15544
• Nom complet: eyes absent homolog 3 (Drosophila)
• Masse moléculaire calculée: 573 aa, 63 kDa
• Poids moléculaire observé: 63-66 kDa
• Numéro d’acquisition GenBank: BC029500
• Symbole du gène: EYA3
• Identification du gène (NCBI): 2140
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EYA3(Eyes absent homolog 3) belongs to the HAD-like hydrolase superfamily.Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis.EYA3, like EYA1, is expressed in the ninth week of human development and may also underlie developmental defects(PMID:9020840).It has 2 isoforms produced by alternative splicing.This antibody is specific to EYA3.

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