Anticorps Recombinant de lapin anti-EZH2

EZH2 Recombinant Antibody for WB, IHC, IF/ICC, ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

242309F11

N° de cat : 84824-2-RR

Synonymes

242309F11, EC:2.1.1.356, Enhancer of zeste homolog 2, ENX 1, ENX-1

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Galerie de données de validation

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  WB analysis using 84824-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84824-2-RR (EZH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 84824-2-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84824-2-RR (EZH2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 84824-2-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84824-2-RR (EZH2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 84824-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EZH2 antibody (84824-2-RR, Clone: 242309F11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  ChIP experiment of NCCIT using 84824-2-RR

  Chromatin was prepared from NCCIT cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of EZH2 (84824-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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  Affinity and Kinetic Characterization of 84824-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84824-2-RR against Human EZH2 were performed. The affinity constant is 2.73 nM.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules C6, cellules NIH/3T3, cellules Raji
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en ChIP-qPCR: cellules NCCIT,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• CHImmunoprécipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84824-2-RR cible EZH2 dans les applications de WB, IHC, IF/ICC, ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: EZH2 Protéine recombinante Ag16789
• Nom complet: enhancer of zeste homolog 2 (Drosophila)
• Masse moléculaire calculée: 751 aa, 86 kDa
• Poids moléculaire observé: 90-102 kDa
• Numéro d’acquisition GenBank: BC010858
• Symbole du gène: EZH2
• Identification du gène (NCBI): 2146
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)

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