Anticorps Recombinant de lapin anti-Endoglin/CD105

Endoglin/CD105 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242305G3

N° de cat : 84807-4-RR

Synonymes

CD105, 242305G3, S endoglin, Eng, endoglin

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Galerie de données de validation

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  WB analysis using 84807-4-RR

  bEnd.3 cells were subjected to SDS PAGE followed by western blot with 84807-4-RR (Endoglin/CD105 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84807-4-RR

  Mouse lung tissue was subjected to SDS PAGE followed by western blot with 84807-4-RR (Endoglin/CD105 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 84807-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84807-4-RR (Endoglin/CD105 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 84807-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84807-4-RR (Endoglin/CD105 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of bEnd.3 using 84807-4-RR

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using Endoglin/CD105 antibody (84807-4-RR, Clone: 242305G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84807-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84807-4-RR against Mouse Endoglin/CD105 were performed. The affinity constant is 0.888 nM.

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Applications testées

• Résultats positifs en WB: cellules bEnd.3, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu rénal de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules bEnd.3,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84807-4-RR cible Endoglin/CD105 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: endoglin
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 85-90 kDa
• Numéro d’acquisition GenBank: NM_007932.2
• Symbole du gène: CD105
• Identification du gène (NCBI): 13805
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodeling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

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