Anticorps Recombinant de lapin anti-Endoglin/CD105

Endoglin/CD105 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242305A11

N° de cat : 84807-5-PBS

Synonymes

CD105, 242305A11, Edg, endoglin, Eng

---

Galerie de données de validation

• 

  WB analysis of bEnd.3 using 84807-5-RR (same clone as 84807-5-PBS)

  bEnd.3 cells were subjected to SDS PAGE followed by western blot with 84807-5-RR (Endoglin/CD105 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84807-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 84807-5-RR (same clone as 84807-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84807-5-RR (Endoglin/CD105 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84807-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 84807-5-RR (same clone as 84807-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84807-5-RR (Endoglin/CD105 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84807-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84807-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84807-5-RR against Mouse Endoglin/CD105 were performed. The affinity constant is 0.766 nM.

---

Informations sur le produit

84807-5-PBS cible Endoglin/CD105 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Endoglin/CD105 Protéine recombinante Eg1292
• Nom complet: endoglin
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: NM_007932.2
• Symbole du gène: CD105
• Identification du gène (NCBI): 13805
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

{{ptg:RelatedPrimaryAntibodies}}