Anticorps Monoclonal anti-Entactin

Entactin Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

2A11G1

Avis (2)

N° de cat : 67310-1-Ig

Synonymes

Entactin, NID, NID 1, NID1, nidogen 1

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Galerie de données de validation

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  WB analysis of mouse kidney using 67310-1-Ig

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67310-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67310-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 67310-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 67310-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 67310-1-Ig

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 67310-1-Ig

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 67310-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67310-1-Ig (Entactin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 67310-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67310-1-Ig (Entactin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human placenta using 67310-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Entactin antibody (67310-1-Ig, Clone: 2A11G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 67310-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Entactin antibody (67310-1-Ig, Clone: 2A11G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HeLa, tissu cardiaque de porc, tissu cardiaque de rat, tissu cardiaque de souris, tissu placentaire humain, tissu rénal de rat
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67310-1-Ig cible Entactin dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Entactin Protéine recombinante Ag4739
• Nom complet: nidogen 1
• Masse moléculaire calculée: 1247 aa, 136 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC045606
• Symbole du gène: Entactin/NID
• Identification du gène (NCBI): 4811
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Avelina (Verified Customer) (04-02-2025)

We titrated 5 different NID1 antibodies and this one performed the best. Current staining appears specific and relevant to what we find in literature.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NHP Placenta

FH

Jillian (Verified Customer) (03-27-2025)

We tested multiple NID1 antibodies and this was the cleanest and best looking one that we recieved

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Placenta

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