Anticorps Recombinant de lapin anti-Entactin

Entactin Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251203H4

N° de cat : 86456-1-PBS

Synonymes

Ent, Nid1, NID-1, Nidogen-1

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Galerie de données de validation

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  WB analysis using 86456-1-RR (same clone as 86456-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86456-1-RR (Entactin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86456-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 86456-1-RR (same clone as 86456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86456-1-RR (Entactin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86456-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 86456-1-RR (same clone as 86456-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Entactin antibody (86456-1-RR, Clone: 251203H4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86456-1-PBS in a different storage buffer formulation.

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Informations sur le produit

86456-1-PBS cible Entactin dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Entactin Protéine recombinante Eg3030
• Nom complet: nidogen 1
• Masse moléculaire calculée: 137 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: NM_010917.3
• Symbole du gène: Nid1
• Identification du gène (NCBI): 18073
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Nidogen 1 (Entactin), first identified from a basement membrane-secreting cell line and the murine EHS tumor, is together with perlecan, laminin, and collagen IV, a ubiquitous component of basement membranes (PMID: 19530222). Nidogen-1 is made by mesenchymal cells and is incorporated into the epithelial BM. It is a sulfated glycoprotein of 150 kDa with three globular domains, G1, G2 and G3, and contains binding sites for other ECM molecules (PMID: 10958695, 10671374).

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