Anticorps Recombinant de lapin anti-EPCAM/CD326

EPCAM/CD326 Recombinant Antibody for Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241243E4

N° de cat : 84073-4-PBS

Synonymes

EPCAM, Adenocarcinoma-associated antigen, CD326, Cell surface glycoprotein Trop-1, CO 17A

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Galerie de données de validation

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  Sandwich ELISA sample test of MP01009-4

  The mean EPCAM/CD326 concentration was determined to be 492922.2 pg/mL in HT-116 cell extract based on a 2.0 mg/mL extract load.

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  Sandwich ELISA sample test of MP01009-4

  HT-29 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human EPCAM/CD326, and measured 6718.5 pg/mL.

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  Sandwich ELISA sample test of MP01009-4

  Serum of six individual healthy human donors was measured. The EPCAM/CD326 concentration of detected samples was determined to be 484.9 pg/mL with a range of 81.5-2002.2 pg/mL.

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  Sandwich ELISA standard curve of MP01009-4

  Sandwich ELISA standard curve of MP01009-4, Human EPCAM/CD326 Recombinant Matched Antibody Pair - PBS only. 84073-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1370. 84073-4-PBS was HRP conjugated as the detection antibody. Range: 19.5-625 pg/mL.

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  Affinity and Kinetic Characterization of 84073-4-PBS

  Biolayer interferometry (BLl) kinetic assay of 84073-4-PBS against Human EpCAM/TROP1 was performed. The affinity constant is 0.622 nM.

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Informations sur le produit

84073-4-PBS cible EPCAM/CD326 dans les applications de Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: EPCAM/CD326 Protéine recombinante Eg1370
• Nom complet: epithelial cell adhesion molecule
• Masse moléculaire calculée: 35 kDa
• Numéro d’acquisition GenBank: NM_002354.3
• Symbole du gène: EPCAM
• Identification du gène (NCBI): 4072
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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