Anticorps Polyclonal de lapin anti-EphA4

• Phare
• Validé par KD/KO

EphA4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21875-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 21875-1-AP (same clone as 21875-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21875-1-AP (EphA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21875-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 21875-1-AP (same clone as 21875-1-PBS)

  IP result of anti-EphA4 (IP:21875-1-AP, 4ug; Detection:21875-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 21875-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21875-1-AP (same clone as 21875-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21875-1-AP (EphA4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21875-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21875-1-AP (same clone as 21875-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21875-1-AP (EphA4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21875-1-PBS in a different storage buffer formulation.

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  IF Staining of Neuron cells using 21875-1-AP (same clone as 21875-1-PBS)

  IF result of EphA4 antibody (21875-1-AP, 1:50) with culture neuron cells by Wilson Pak Kin Lou. This data was developed using the same antibody clone with 21875-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21875-1-PBS cible EphA4 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EphA4 Protéine recombinante Ag16233
• Nom complet: EPH receptor A4
• Masse moléculaire calculée: 986 aa, 110 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC026327
• Symbole du gène: EPHA4
• Identification du gène (NCBI): 2043
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EphA4 is a member of the Eph receptor tyrosine kinase family and has important functions in the developing and adult nervous system (PMID: 14697664). The Eph receptors comprise a large family of closely related transmembrane tyrosine kinases that actively signal when bound to their ephrin ligands. The Eph receptors are characterized by an extracellular region with a unique cysteine-rich motif extending over its amino-terminal half, followed by two fibronectin type III motifs (PMID: 9530499). They are divided into two sub-groups (EphA and EphB) based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands (PMID: 11114742). EphA4 is involved in commissure formation within the forebrain, axonal guidance in the corticospinal tract, regulation of the central pattern generator that provides normal locomotor function and axonal regeneration following spinal cord injury (PMID: 30061574). EphA4 has been implicated as a disease modifier of amyotrophic lateral sclerosis (ALS) (PMID: 22922411).

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