Anticorps Polyclonal de lapin anti-Ephrin B2

Ephrin B2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 26533-1-PBS

Synonymes

EFNB2, EPLG5, HTK ligand, Htk L, Ephrin-B2

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Galerie de données de validation

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  WB analysis using 26533-1-AP (same clone as 26533-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26533-1-AP (Ephrin B2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26533-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 26533-1-AP (same clone as 26533-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Ephrin B2 antibody (26533-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 26533-1-PBS in a different storage buffer formulation.

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Informations sur le produit

26533-1-PBS cible Ephrin B2 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Ephrin B2 Protéine recombinante Ag24865
• Nom complet: ephrin-B2
• Masse moléculaire calculée: 333 aa, 37 kDa
• Poids moléculaire observé: 47-50 kDa
• Numéro d’acquisition GenBank: BC074856
• Symbole du gène: EFNB2
• Identification du gène (NCBI): 1948
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ephrin B2 (EFNB2) is a transmembrane ligand for the Ephrin B class (EPHB) of receptor tyrosine kinases. EPHB receptor(EPHB1-EPHB4, EPH6) are located at the member of one cell and activated by binding EFNB protein located on another cell, resulting in a bidirectional Ephrin-EphR signaling (PMID: 26790531). Ephrin-B are involved in vasculogenesis, neural development and synaptic plasticity, and in pathological processes such as cancer or neurodegeneration (PMID:29508392). The calculated molecular weight of EFNB2 is 37 kDa, and there is also detected about 50 kDa band in the article (PMID: 27650867, PMID: 23492730).

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