Anticorps Monoclonal anti-Estradiol

Estradiol Monoclonal Antibody for ELISA, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Estradiol

Applications

ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E5E4

N° de cat : 68600-1-PBS

Synonymes

17 beta oestradiol, beta Estradiol, C18H24O2, cis-Estradiol, Estradiol, Estradiol hemihydrate, Estradiolum

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Galerie de données de validation

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  ELISA experiment of Estradiol using 68600-1-Ig (same clone as 68600-1-PBS)

  Indirect ELISA was performed by coating BSA-Estradiol ~30 ng/well (by Estradiol amount), followed by blocking with 1% BSA. Serial diluted Estradiol antibody 68600-1-Ig was added to the plates and incubated at 37℃. HRP-Goat anti-mouse was used for detection (top X-right Y, blue curve). Competitive ELISA was performed similarly except that different concentration of Estradiol was mixed in 30ng/mL primary antibody (bottom X-left Y, red curve). This data was developed using the same antibody clone with 68600-1-PBS in a different storage buffer formulation.

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  ELISA experiment of Estradiol using 68600-1-PBS

  BSA-Estradiol was coated in 96 well plate at ~30 ng/well (by Estradiol amount), followed by blocking with 1% BSA. Different concentrations of Estradiol standard as well as diluted urine from different donor were mixed with 30 ng/mL Estradiol antibody 68600-1-Ig respectively. Urine Estradiol was calculated based on standard curve. Please notice that the level from different donor might be different between individuals. This data was developed using the same antibody clone with 68600-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68600-1-PBS cible Estradiol dans les applications de ELISA, Indirect ELISA et montre une réactivité avec des échantillons Estradiol

• Réactivité: Estradiol
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PTG
• Nom complet: Estradiol
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Estradiol is a naturally occurring hormone circulating endogenously in females. It is commercially available in several hormone therapy products for managing conditions associated with reduced estrogen, such as vulvovaginal atrophy and hot flashes. Some available forms of estradiol include oral tablets, injections, vaginal rings, transdermal patches, sprays, gels, and creams. This antibody can be used in the Elisa experiments.

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