Anticorps Recombinant de lapin anti-Exportin 4

• Phare
• Validé par KD/KO

Exportin 4 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241443A5

N° de cat : 84449-1-RR

Synonymes

XPO4, 241443A5, Exp4, Exportin-4, KIAA1721

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Galerie de données de validation

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  WB analysis using 84449-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84449-1-RR (XPO4 antibody) at dilution of 1:8500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 84449-1-RR

  WB result of Exportin 4 antibody (84449-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Exportin 4 transfected HEK-293 cells.

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  IF Staining of K-562 using 84449-1-RR

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using Exportin 4 antibody (84449-1-RR, Clone: 241443A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 84449-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Exportin 4 Recombinant antibody (84449-1-RR, Clone:241443A5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 84449-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84449-1-RR against Human Exportin 4 were performed. The affinity constant is 0.512 nM.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules K-562, cellules RAW 264.7, cellules SW480
• Résultats positifs en IF/ICC: cellules K-562,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:17000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84449-1-RR cible Exportin 4 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Exportin 4 Protéine recombinante Ag21845
• Nom complet: exportin 4
• Masse moléculaire calculée: 1151 aa, 130 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC113571
• Symbole du gène: Exportin 4/XPO4
• Identification du gène (NCBI): 64328
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

exportin 4 (XPO4) maps to chromosome 13q and belongs to the importin β family which acts as a bidirectional nuclear transport receptor. Decreased XPO4 expression has been shown to associate with hepatocellular carcinoma. A genome-wide copy number variation (CNV) association study in a small cohort of Malaysian subjects identified a CNV in XPO4 to be correlated with the progression of metabolic (dysfunction) associated fatty liver disease (MAFLD). (PMID: 34388518)

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