Anticorps Polyclonal de lapin anti-JAM-A/CD321

JAM-A/CD321 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 16183-1-AP

Synonymes

F11R, JAM-A/Junctional Adhesion Molecule A, JAM A, JAM 1, JAM

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Galerie de données de validation

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  WB analysis of mouse liver using 16183-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16183-1-AP (JAM-A/Junctional Adhesion Molecule A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16183-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16183-1-AP (JAM-A/Junctional Adhesion Molecule A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 16183-1-AP

  IP result of anti-JAM-A/Junctional Adhesion Molecule A (IP:16183-1-AP, 4ug; Detection:16183-1-AP 1:300) with mouse liver tissue lysate 3000ug.

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  IHC staining of human breast cancer using 16183-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16183-1-AP (JAM-A/Junctional Adhesion Molecule A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16183-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16183-1-AP (JAM-A/Junctional Adhesion Molecule A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, plaquettes de sang périphérique humain
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

16183-1-AP cible JAM-A/CD321 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: F11 receptor
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC001533
• Symbole du gène: F11R
• Identification du gène (NCBI): 50848
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The F11 receptor (F11R) was first identified in human platelets as a 32/35 kDa protein duplex that serves as the receptor for a functional antibody that activates platelets. It seems to play a role in epithelial tight junction formation. It was also reported to function as a receptor for reovirus and a ligand for the integrin LFA1.

Publications

Species	Application	Title
human	WB,IHC	Front Oncol; The Long Non-Coding RNA HOXC-AS3 Promotes Glioma Progression by Sponging miR-216 to Regulate F11R Expression.; Authors - Yongshuai Li
human	WB	Clin Exp Pharmacol Physiol; Rabeprazole destroyed gastric epithelial barrier function through FOXF1/STAT3-mediated ZO-1 expression; Authors - Fangying Yang
rat	WB	J Chem Neuroanat; Matrine treatment induced an A2 astrocyte phenotype and protected the blood-brain barrier in CNS autoimmunity.; Authors - Yilei Jing
human	WB	Biomedicines; Identification and Validation of JAM-A as a Novel Prognostic and Immune Factor in Human Tumors; Authors - Tianyi Ren
mouse	WB	Int J Biol Macromol; Epicatechin and β-glucan from whole highland barley grain ameliorates hyperlipidemia associated with attenuating intestinal barrier dysfunction and modulating gut microbiota in high-fat-diet-fed mice; Authors - Zehua Liu

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