Anticorps Polyclonal de lapin anti-F4/80

F4/80 Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

31

N° de cat : 29414-1-AP

Synonymes

Adgre1, DD7A5 7, EGF TM7, Emr1, F4 80, F4/80, Gpf480, Ly71, TM7LN3

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Galerie de données de validation

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  IHC staining of mouse liver using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:12000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:12000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:12000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:12000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 29414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 29414-1-AP (F4/80 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 29414-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (29414-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse spleen using 29414-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (29414-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu splénique de souris, tissu cardiaque de souris, tissu cérébral de souris, tissu d'intestin grêle de souris, tissu hépatique de rat, tissu hépatique de souris, tissu pulmonaire de souris, tissu splénique de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu splénique de souris,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:8000-1:20000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 17 publications below
• IF: See 15 publications below

Informations sur le produit

29414-1-AP cible F4/80 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: F4/80 Protéine recombinante Ag30149
• Nom complet: EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• Masse moléculaire calculée: 102 kDa
• Numéro d’acquisition GenBank: NM_010130
• Symbole du gène: Emr1
• Identification du gène (NCBI): 13733
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mouse F4/80, also named as EMR1 and Gpf480, is a 160kd cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.

Publications

Species	Application	Title
human	IHC,IF	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
mouse	IHC,IF	J Exp Clin Cancer Res; FAM171B stabilizes vimentin and enhances CCL2-mediated TAM infiltration to promote bladder cancer progression; Authors - Wei-Min Hu
rat	IHC,IF	Mater Today Bio; Mussel-inspired collagen-hyaluronic acid composite scaffold with excellent antioxidant properties and sustained release of a growth factor for enhancing diabetic wound healing.; Authors - Yong Wang
mouse	IF	ACS Appl Mater Interfaces; Catalysis-Mediated Male Contraception through Black Phosphorus Nanosheets; Authors - Lirong Yi
rat	IF	Mater Today Bio; Intracellular hydrogelation of macrophage conjugated probiotics for hitchhiking delivery and combined treatment of colitis; Authors - Jingzhe Wang
mouse	IF	Int Immunopharmacol; The ALOX5 inhibitor Zileuton regulates tumor-associated macrophage M2 polarization by JAK/STAT and inhibits pancreatic cancer invasion and metastasis; Authors - Wei-Min Hu