Anticorps Recombinant de lapin anti-F4/80

F4/80 Recombinant Antibody for IHC, IF/ICC, IF-P, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

IHC, IF/ICC, IF-P, IF-Fro, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

2P18

N° de cat : 81668-1-RR

Synonymes

Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1

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Galerie de données de validation

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  IHC staining of mouse spleen using 81668-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 81668-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 81668-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 81668-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 81668-1-RR

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (81668-1-RR, Clone: 2P18 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse spleen using 81668-1-RR

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (81668-1-RR, Clone: 2P18 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse spleen using 81668-1-RR

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse spleen tissue using F4/80 antibody (81668-1-RR, Clone: 2P18 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of RAW 264.7 using 81668-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using F4/80 antibody (81668-1-RR, Clone: 2P18 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 81668-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81668-1-RR against Mouse F4/80 were performed. The affinity constant is 14.4 nM.

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Applications testées

• Résultats positifs en IHC: tissu splénique de souris, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu splénique de souris, cellules RAW 264.7
• Résultats positifs en IF-Fro: tissu splénique de souris,
• Résultats positifs en IF/ICC: cellules RAW 264.7,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:2000-1:3000
• Immunofluorescence (IF)-P: IF-P : 1:750-1:3000
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below

Informations sur le produit

81668-1-RR cible F4/80 dans les applications de IHC, IF/ICC, IF-P, IF-Fro, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: F4/80 Protéine recombinante Ag28252
• Nom complet: EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• Masse moléculaire calculée: 102 kDa
• Numéro d’acquisition GenBank: NM_010130
• Symbole du gène: F4/80
• Identification du gène (NCBI): 13733
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mouse F4/80, also named as EMR1 and Gpf480, is a 160kd cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.

Publications

Species	Application	Title
mouse	IF	bioRxiv; Integrin alpha1 beta1 promotes interstitial fibrosis in a mouse model of polycystic kidney disease; Authors - C Grenier
		Genes Immun; Annexin A1 regulates inflammatory-immune response and reduces pancreatic and extra- pancreatic injury during severe acute pancreatitis; Authors - Shizhao Lin
mouse	IF	Cell Rep; The rebalancing of the immune system at the maternal-fetal interface ameliorates autism-like behavior in adult offspring; Authors - Chunxiang Shen

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