Anticorps Polyclonal de lapin anti-FAAH

• Phare
• Validé par KD/KO

FAAH Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 17909-1-AP

Synonymes

Anandamide amidohydrolase 1, FAAH, FAAH 1, FAAH1, fatty acid amide hydrolase, Fatty acid amide hydrolase 1, Oleamide hydrolase 1

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Galerie de données de validation

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  WB analysis using 17909-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17909-1-AP (FAAH antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 17909-1-AP

  WB result of FAAH antibody (17909-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAAH transfected A431 cells.

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  WB analysis of rat testis using 17909-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 17909-1-AP (FAAH Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17909-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17909-1-AP (FAAH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 17909-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17909-1-AP (FAAH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 17909-1-AP

  IP result of anti-FAAH (IP:17909-1-AP, 4ug; Detection:17909-1-AP 1:500) with mouse liver tissue lysate 6400 ug.

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  IHC staining of mouse testis using 17909-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17909-1-AP (FAAH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17909-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17909-1-AP (FAAH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17909-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17909-1-AP (FAAH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17909-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17909-1-AP (FAAH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 17909-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17909-1-AP (FAAH Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 17909-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17909-1-AP (FAAH Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules A431, tissu testiculaire de rat
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu testiculaire de souris, tissu placentaire humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:300

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

17909-1-AP cible FAAH dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FAAH Protéine recombinante Ag12304
• Nom complet: fatty acid amide hydrolase
• Masse moléculaire calculée: 579 aa, 63 kDa
• Poids moléculaire observé: 55-65 kDa
• Numéro d’acquisition GenBank: BC093632
• Symbole du gène: FAAH
• Identification du gène (NCBI): 2166
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fatty acid amide hydrolase (FAAH) is a membrane-bound homodimeric enzyme that in vivo controls content and biological activity of N-arachidonoylethanolamine (AEA) and other relevant bioactive lipids termed endocannabinoids. (PMID: 32041998) This antibody is specific for FAAH protein.

Publications

Species	Application	Title
mouse	WB	Elife; p21-activated kinase 1 restricts tonic endocannabinoid signaling in the hippocampus.; Authors - Shuting Xia
rat	WB	J Proteome Res; Joint Synovial Fluid Metabolomics Method to Decipher the Metabolic Mechanisms of Adjuvant Arthritis and Geniposide Intervention.; Authors - Xiang Zhan
mouse	WB	Neuroscience; The Endocannabinoid System Contributes to Memory Deficits Induced by Rapid-eye-movement Sleep Deprivation in Adolescent Mice.; Authors - Jinming Zhang
human,mouse	WB,IP	Cell Death Differ; FAAH served a key membrane-anchoring and stabilizing role for NLRP3 protein independently of the endocannabinoid system; Authors - Yangyang Zhu ; KO Validated
human	WB	Cell Death Dis; Combination treatment with FAAH inhibitors/URB597 and ferroptosis inducers significantly decreases the growth and metastasis of renal cell carcinoma cells via the PI3K-AKT signaling pathway; Authors - Junfeng Hao