Anticorps Monoclonal anti-FABP1

FABP1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B6C8

N° de cat : 68227-1-PBS

Synonymes

liver FABP, 1B6C8, FABPL, Fatty acid binding protein 1, Fatty acid-binding protein 1

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Galerie de données de validation

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  WB analysis using 68227-1-Ig (same clone as 68227-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68227-1-Ig (FABP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 68227-1-Ig (same clone as 68227-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68227-1-Ig (FABP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 68227-1-Ig (same clone as 68227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68227-1-Ig (FABP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 68227-1-Ig (same clone as 68227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68227-1-Ig (FABP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 68227-1-Ig (same clone as 68227-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using FABP1 antibody (68227-1-Ig, Clone: 1B6C8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 68227-1-Ig (same clone as 68227-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using FABP1 antibody (68227-1-Ig, Clone: 1B6C8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 68227-1-Ig (same clone as 68227-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug FABP1 Monoclonal antibody (68227-1-Ig, Clone:1B6C8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68227-1-PBS cible FABP1 dans les applications de WB, IHC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FABP1 Protéine recombinante Ag32836
• Nom complet: fatty acid binding protein 1, liver
• Masse moléculaire calculée: 127 aa, 14 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC032801
• Symbole du gène: FABP1
• Identification du gène (NCBI): 2168
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FABP1, liver fatty acid-binding protein, is abundant in cytoplasm that regulates lipid transport and metabolism. It plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (PMID:25732850). FABP1 is mainly expressed in hepatocytes, enterocytes and to a lesser degree in renal tubular cells, associated with liver injury (PMID: 15653098). Levels of FABP1 was elevated in patients with hepatocyte injury secondary to alcohol or drug toxicity (PMID: 14563446).

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