Anticorps Monoclonal anti-FABP2

• Phare
• Validé par KD/KO

FABP2 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

2D11G6

N° de cat : 67691-1-Ig

Synonymes

2D11G6, FABPI, Fatty acid binding protein 2, Fatty acid-binding protein 2, Fatty acid-binding protein, intestinal

---

Galerie de données de validation

• 

  WB analysis using 67691-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67691-1-Ig (FABP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 67691-1-Ig

  WB result of FABP2 antibody (67691-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FABP2 transfected COLO 320 cells.

• 

  WB analysis using 67691-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67691-1-Ig (FABP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat small intestine using 67691-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 67691-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 67691-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 67691-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 67691-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 67691-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse colon using 67691-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using FABP2 antibody (67691-1-Ig, Clone: 2D11G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Applications testées

• Résultats positifs en WB: tissu d'intestin grêle de rat, cellules COLO 320, duodénum de porc, intestin grêle de lapin, intestin grêle de souris, tissu de jéjunum humain
• Résultats positifs en IHC: tissu d'intestin grêle de souris, tissu de côlon de souris, tissu d'intestin grêle de rat, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de côlon de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:50000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

67691-1-Ig cible FABP2 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FABP2 Protéine recombinante Ag17620
• Nom complet: fatty acid binding protein 2, intestinal
• Masse moléculaire calculée: 132 aa, 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC069617
• Symbole du gène: FABP2
• Identification du gène (NCBI): 2169
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FABP2, also known as the intestinal fatty acid binding protein (I-FABP), is expressed in the absorptive intestinal villus cells. It is mainly involved in intracellular transport and intestinal absorption of lipids. FABP2 has been considered a marker of mucosal injury and ischemia and serum I-FABP level is used as a tissue damage indicator. In addition, it is a marker of differentiated intestinal epithelial cells.

Publications

Species	Application	Title
mouse	WB	Int J Mol Sci; Anti-Proteolytic Peptide R7I Protects the Intestinal Barrier and Alleviates Fatty Acid Malabsorption in Salmonella typhimurium-Infected Mice; Authors - Yunzhe Su
mouse	IF	Gut Microbes; Infant feces-derived Lactobacillus gasseri FWJL-4 mitigates experimental necrotizing enterocolitis via acetate production; Authors - Li-Long Pan
	WB	Cell Biochem Funct; Neutrophil Depletion Reduced the Relative Abundance of Unsaturated Long-Chain Fatty Acid Synthesis Microbiota and Intestinal Lipid Absorption; Authors - Xingyu Lu

{{ptg:RelatedPrimaryAntibodies}}