Anticorps Polyclonal de lapin anti-FABP3

• Phare
• Validé par KD/KO

FABP3 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

19

Avis (1)

N° de cat : 10676-1-AP

Synonymes

FABP11, FABP3, Fatty acid binding protein 3, H FABP, HFABP, M FABP, MDGI, O FABP

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Galerie de données de validation

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  WB analysis using 10676-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10676-1-AP (FABP3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 10676-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 10676-1-AP (FABP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 10676-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10676-1-AP (FABP3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 10676-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10676-1-AP (FABP3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse heart using 10676-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10676-1-AP (FABP3 antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of mouse heart using 10676-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10676-1-AP (FABP3 antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human skin using 10676-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10676-1-AP (FABP3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 10676-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 10676-1-AP (FABP3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 10676-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 10676-1-AP (FABP3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 10676-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10676-1-AP (FABP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 10676-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10676-1-AP (FABP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse brain using 10676-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FABP3 antibody (10676-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 10676-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FABP3 antibody (10676-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de rat, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris, tissu testiculaire de rat
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cardiaque humain, tissu cutané humain, tissu de cancer du sein humain, tissu ovarien humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Informations sur le produit

10676-1-AP cible FABP3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FABP3 Protéine recombinante Ag1069
• Nom complet: fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor)
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC007021
• Symbole du gène: FABP3
• Identification du gène (NCBI): 2170
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FABP3 (fatty-acid-binding protein 3), also known as heart-type FABP or mammary-derived growth inhibitor (MDGI), is a small 15-kDa cytoplasmic protein transporting fatty acids and other lipophilic substances from the cytoplasm to the nucleus. It is most ubiquitously expressed in heart and skeletal muscle.

Publications

Species	Application	Title
mouse	WB	ACS Nano; Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy; Authors - Qiqi Zhang
mouse	WB	Biomaterials; MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.; Authors - Mingming Song
mouse	WB	J Neurosci; FABP3 in the Anterior Cingulate Cortex Modulates the Methylation Status of the Glutamic Acid Decarboxylase67 Promoter Region.; Authors - Yui Yamamoto
mouse	WB	Int J Mol Sci; Amelioration of Nicotine-Induced Conditioned Place Preference Behaviors in Mice by an FABP3 Inhibitor; Authors - Wenbin Jia
mouse	WB	J Cell Mol Med; PMID: 21251210; Authors - Xi Lei L
mouse	WB,IF	J Biol Chem; FABP3 promotes αSyn oligomerization associated with MPTP-induced neurotoxicity.; Authors - Norifumi Shioda ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sampath Kumar (Verified Customer) (02-26-2024)

The FABP3 antibody worked well at 1:1000 dilution on rodent skeletal muscle tissue homogenates (6ug total protein), producing bands at 15kDa. However, homogenates from Avian pectoralis muscle homogenates (6ug total protein) produced barely visible bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Avian and rodent skeletal muscle

FABP3 Antibody Western Blot validation (1:1000 dilution) in Avian and rodent skeletal muscle (Cat no:10676-1-AP)