Anticorps Monoclonal anti-FABP3

FABP3 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

2B5C1

Avis (1)

N° de cat : 60280-1-Ig

Synonymes

FABP11, FABP3, Fatty acid binding protein 3, H FABP, M FABP, MDGI, O FABP

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Galerie de données de validation

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  WB analysis using 60280-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60280-1-Ig (FABP3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 60280-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60280-1-Ig (FABP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 60280-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60280-1-Ig (FABP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 60280-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using FABP3 antibody (60280-1-Ig, Clone: 2B5C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse heart using 60280-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using FABP3 antibody (60280-1-Ig, Clone: 2B5C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse heart using 60280-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using FABP3 antibody (60280-1-Ig, Clone: 2B5C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain, cœur de rat, cœur de souris
• Résultats positifs en IHC: tissu cardiaque humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1900-1:7600
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

60280-1-Ig cible FABP3 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FABP3 Protéine recombinante Ag21483
• Nom complet: fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor)
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC007021
• Symbole du gène: FABP3
• Identification du gène (NCBI): 2170
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FABP3 (fatty-acid-binding protein 3), also known as heart-type FABP or mammary-derived growth inhibitor (MDGI), is a small 15-kDa cytoplasmic protein transporting fatty acids and other lipophilic substances from the cytoplasm to the nucleus. It is most ubiquitously expressed in heart and skeletal muscle.

Publications

Species	Application	Title
human	IF	J Cell Mol Med; Four transcription profile-based models identify novel prognostic signatures in oesophageal cancer.; Authors - Tongyan Liu
human	WB	Mol Cell Proteomics; Quantitative ubiquitylome analysis reveals specificity of RNF111/Arkadia E3 ubiquitin ligase for its degradative substrates SKI and SKIL/SnoN in TGF-β signaling pathway; Authors - Victor Laigle
human	WB	Front Oncol; Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma.; Authors - Jinghui Lu
mouse	WB	Mol Med; Calpain inhibition as a novel therapeutic strategy for aortic dissection with acute lower extremity ischemia; Authors - Qiwen Tan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marco (Verified Customer) (07-04-2024)

We are very happy with the results of this antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NRVCMs

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