Anticorps Polyclonal de lapin anti-FABP4

• Phare
• Validé par KD/KO

FABP4 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

91

Avis (1)

N° de cat : 12802-1-AP

Synonymes

A FABP, AFABP, ALBP, aP2, FABP4, Fatty acid binding protein 4

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Galerie de données de validation

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  WB analysis using 12802-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12802-1-AP (FABP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 12802-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12802-1-AP (FABP4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 12802-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12802-1-AP (FABP4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat brown adipose using 12802-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brown adipose slide using 12802-1-AP (FABP4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brown adipose using 12802-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brown adipose slide using 12802-1-AP (FABP4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 12802-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12802-1-AP (FABP4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 12802-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12802-1-AP (FABP4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12802-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12802-1-AP (FABP4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 12802-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12802-1-AP (FABP4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 12802-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12802-1-AP (FABP4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12802-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12802-1-AP (FABP4 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, cœur de rat, muscle squelettique de souris, tissu adipeux de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cardiaque humain, tissu cutané de souris, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 77 publications below
• IHC: See 16 publications below
• IF: See 10 publications below

Informations sur le produit

12802-1-AP cible FABP4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris, Hamster, Oie
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FABP4 Protéine recombinante Ag3912
• Nom complet: fatty acid binding protein 4, adipocyte
• Masse moléculaire calculée: 132 aa, 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC003672
• Symbole du gène: FABP4
• Identification du gène (NCBI): 2167
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fatty acid binding protein (FABP) 4 is a member of the FABP family which abundantly expressed, fatty acid carrier proteins. FABPs are capable of binding a variety of hydrophobic molecules such as long-chain fatty acids and are important for their uptake and intracellular trafficking. It was first identified as an adipocyte-specific protein, important for the maintenance of lipid and glucose metabolism. It is also detected in macrophages, where it participates in regulating inflammation and cholesterol trafficking via NFκB and PPAR. In more recent studies, FABP4 has been found in a variety of endothelial cells, where it has been identified as a target of VEGF and a regulator of cell proliferation and possibly angiogenesis. Pathologically, FABP4 has been associated with the development of metabolic syndrome, diabetes and cancer and vulnerability of atherosclerotic plaques. FABP4 has been identified as a novel prognostic factor for both adverse cardiovascular events and breast cancer.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Tip60-mediated lipin 1 acetylation and ER translocation determine triacylglycerol synthesis rate.; Authors - Terytty Yang Li
mouse	WB,IF	Biomaterials; MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.; Authors - Mingming Song
mouse	WB	Cell Rep; Uncoupling of Metabolic Health from Longevity through Genetic Alteration of Adipose Tissue Lipid-Binding Proteins.; Authors - Khanichi N Charles
human	WB,IF	Cell Mol Immunol; PMID: 31363171; Authors - Tian-Yu Lin
human	WB	Stem Cells; miR-223 regulates adipogenic and osteogenic differentiation of mesenchymal stem cells through a C/EBPs/miR-223/FGFR2 regulatory feedback loop.; Authors - Xiaohui Guan
mouse	WB	Diabetes; Pygo2 Regulates Adiposity and Glucose Homeostasis via β-Catenin-Axin2-GSK3β Signaling Pathway.; Authors - Yuan-Yuan Xie

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Huai-Chin (Verified Customer) (05-20-2022)

Western blot on 3T3-L1 cells pre and post adipogenesis. Works pretty well. Strong band in 3T3-L1 cells after adipogenesis.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10,000
• Cell Tissue Type: 3T3-L1

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