Anticorps Polyclonal de lapin anti-FABP4

• Phare
• Validé par KD/KO

FABP4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12802-1-PBS

Synonymes

Adipocyte lipid-binding protein, Adipocyte-type fatty acid-binding protein, AFABP, ALBP, Fatty acid binding protein 4

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Galerie de données de validation

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  WB analysis of MC38 using 12802-1-AP (same clone as 12802-1-PBS)

  WB result of FABP4 antibody (12802-1-AP; 1:800; room temperature for 1.5 hours) with wild-type and FABP4 knockout MC38 cells. This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

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  WB analysis using 12802-1-AP (same clone as 12802-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12802-1-AP (FABP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brown adipose using 12802-1-AP (same clone as 12802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brown adipose slide using 12802-1-AP (FABP4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brown adipose using 12802-1-AP (same clone as 12802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brown adipose slide using 12802-1-AP (FABP4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 12802-1-AP (same clone as 12802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12802-1-AP (FABP4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 12802-1-AP (same clone as 12802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12802-1-AP (FABP4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 12802-1-AP (same clone as 12802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12802-1-AP (FABP4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 12802-1-AP (same clone as 12802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12802-1-AP (FABP4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 12802-1-AP (same clone as 12802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 12802-1-AP (FABP4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 12802-1-AP (same clone as 12802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 12802-1-AP (FABP4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 12802-1-AP (same clone as 12802-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using FABP4 antibody (12802-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12802-1-PBS cible FABP4 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FABP4 Protéine recombinante Ag3912
• Nom complet: fatty acid binding protein 4, adipocyte
• Masse moléculaire calculée: 132 aa, 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC003672
• Symbole du gène: FABP4
• Identification du gène (NCBI): 2167
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Fatty acid binding protein (FABP) 4 is a member of the FABP family which abundantly expressed, fatty acid carrier proteins. FABPs are capable of binding a variety of hydrophobic molecules such as long-chain fatty acids and are important for their uptake and intracellular trafficking. It was first identified as an adipocyte-specific protein, important for the maintenance of lipid and glucose metabolism. It is also detected in macrophages, where it participates in regulating inflammation and cholesterol trafficking via NFκB and PPAR. In more recent studies, FABP4 has been found in a variety of endothelial cells, where it has been identified as a target of VEGF and a regulator of cell proliferation and possibly angiogenesis. Pathologically, FABP4 has been associated with the development of metabolic syndrome, diabetes and cancer and vulnerability of atherosclerotic plaques. FABP4 has been identified as a novel prognostic factor for both adverse cardiovascular events and breast cancer.

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