Anticorps Polyclonal de lapin anti-FABP4

• Phare
• Validé par KD/KO

FABP4 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 31129-1-AP

Synonymes

Adipocyte lipid-binding protein, Adipocyte-type fatty acid-binding protein, AFABP, ALBP, Fatty acid binding protein 4

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Galerie de données de validation

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  WB analysis of MC38 using 31129-1-AP

  WB result of FABP4 antibody (31129-1-AP; 1:400; room temperature for 1.5 hours) with wild-type and FABP4 knockout MC38 cells.

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  WB analysis using 31129-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31129-1-AP (FABP4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of 3T3-L1 using 31129-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed 3T3-L1 cells using FABP4 antibody (31129-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu cardiaque de rat, tissu de muscle squelettique de rat
• Résultats positifs en IF/ICC: cellules 3T3-L1,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

31129-1-AP cible FABP4 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FABP4 Protéine recombinante Eg0880
• Nom complet: fatty acid binding protein 4, adipocyte
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: NM_001442.3
• Symbole du gène: FABP4
• Identification du gène (NCBI): 2167
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fatty acid binding protein (FABP) 4 is a member of the FABP family which abundantly expressed, fatty acid carrier proteins. FABPs are capable of binding a variety of hydrophobic molecules such as long-chain fatty acids and are important for their uptake and intracellular trafficking. It was first identified as an adipocyte-specific protein, important for the maintenance of lipid and glucose metabolism. It is also detected in macrophages, where it participates in regulating inflammation and cholesterol trafficking via NFκB and PPAR. In more recent studies, FABP4 has been found in a variety of endothelial cells, where it has been identified as a target of VEGF and a regulator of cell proliferation and possibly angiogenesis. Pathologically, FABP4 has been associated with the development of metabolic syndrome, diabetes and cancer and vulnerability of atherosclerotic plaques. FABP4 has been identified as a novel prognostic factor for both adverse cardiovascular events and breast cancer.

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