Anticorps Monoclonal anti-FABP4

FABP4 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

3E7E1

N° de cat : 67167-1-Ig

Synonymes

A FABP, AFABP, ALBP, aP2, FABP4, Fatty acid binding protein 4

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Galerie de données de validation

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  WB analysis using 67167-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67167-1-Ig (FABP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67167-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67167-1-Ig (FABP4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de rat, tissu adipeux de porc, tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:800-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

67167-1-Ig cible FABP4 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FABP4 Protéine recombinante Ag8565
• Nom complet: fatty acid binding protein 4, adipocyte
• Masse moléculaire calculée: 132 aa, 15 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC003672
• Symbole du gène: FABP4
• Identification du gène (NCBI): 2167
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fatty acid binding protein (FABP) 4 is a member of the FABP family which abundantly expressed, fatty acid carrier proteins. FABPs are capable of binding a variety of hydrophobic molecules such as long-chain fatty acids and are important for their uptake and intracellular trafficking. It was first identified as an adipocyte-specific protein, important for the maintenance of lipid and glucose metabolism. It is also detected in macrophages, where it participates in regulating inflammation and cholesterol trafficking via NFκB and PPAR. In more recent studies, FABP4 has been found in a variety of endothelial cells, where it has been identified as a target of VEGF and a regulator of cell proliferation and possibly angiogenesis. Pathologically, FABP4 has been associated with the development of metabolic syndrome, diabetes and cancer and vulnerability of atherosclerotic plaques. FABP4 has been identified as a novel prognostic factor for both adverse cardiovascular events and breast cancer.

Publications

Species	Application	Title
mouse	WB	Stem Cell Res Ther; Oncostatin M receptor regulates osteoblast differentiation via extracellular signal-regulated kinase/autophagy signaling.; Authors - Jie Zhou
mouse	IHC	Int J Biol Sci; LAMC1-mediated preadipocytes differentiation promoted peritoneum pre-metastatic niche formation and gastric cancer metastasis.; Authors - Yan Fang
human	WB	Front Oncol; Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma.; Authors - Jinghui Lu
mouse	WB	Biol Pharm Bull; Sorghum (Sorghum bicolor) Extract-Induced Adipogenesis Is Independent of PPARγ Ser273 Phosphorylation in 3T3-L1 Adipocytes.; Authors - Aozora Nagaoka