Anticorps Polyclonal de lapin anti-FABP5

• Phare
• Validé par KD/KO

FABP5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(46)

Avis (1)

N° de cat : 12348-1-AP

Synonymes

EFABP, Epidermal-type fatty acid-binding protein, Fatty acid binding protein 5, Fatty acid-binding protein 5, Fatty acid-binding protein, epidermal

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Galerie de données de validation

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  WB analysis of A375 using 12348-1-AP

  WB result of FABP5 antibody (12348-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FABP5 transfected A375 cells.

• 

  WB analysis using 12348-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12348-1-AP (FABP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Hepa1-6 using 12348-1-AP

  WB result of FABP5 antibody (12348-1-AP; 1:800; room temperature for 1.5 hours) with wild-type and FABP5 knockout Hepa1-6 cells.

• 

  WB analysis of mouse skin using 12348-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 12348-1-AP (FABP5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A375 using 12348-1-AP

  IP result of anti-FABP5 (IP:12348-1-AP, 4ug; Detection:12348-1-AP 1:4000) with A375 cells lysate 1520 ug.

• 

  IHC staining of mouse skin using 12348-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12348-1-AP (FABP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 12348-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12348-1-AP (FABP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12348-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FABP5 antibody (12348-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  FC experiment of HeLa using 12348-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug FABP5 Polyclonal antibody (12348-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules A431, cellules HeLa, cellules Hepa1-6, cellules HepG2, tissu cutané de souris
• Résultats positifs en IP: cellules A375,
• Résultats positifs en IHC: tissu cutané de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 37 publications below
• IHC: See 12 publications below
• IF: See 11 publications below
• CoIP: See 3 publications below

Informations sur le produit

12348-1-AP cible FABP5 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FABP5 Protéine recombinante Ag3005
• Nom complet: fatty acid binding protein 5 (psoriasis-associated)
• Masse moléculaire calculée: 135 aa, 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC019385
• Symbole du gène: FABP5
• Identification du gène (NCBI): 2171
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FABP5, also named as PA-FABP and E-FABP, belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. It is high specificity for fatty acids. FABP5 is highest affinity for C18 chain length. It may be involved in keratinocyte differentiation. FABP5 is a fatty acid-binding protein and is expressed in epidermis and endothelial cells of the microvasculature of different organs. FABP5 has also been identified as a tumor-associated antigen, which is highly expressed in various cancers. FABP5 was detected in the sera of HNSCC patients with early stage cancer. Antibodies specific for FABP5 were significantly increased in a substantial amount in patients, suggesting that FABP5 may be a potential diagnostic biomarker for HNSCC. FABP5 may serve as a biomarker for HNSCC.(PMID:19602232)

Publications

Species	Application	Title
mouse	CoIP	Nat Metab; Intestinal TM6SF2 protects against metabolic dysfunction-associated steatohepatitis through the gut-liver axis; Authors - Xiang Zhang
mouse,human	WB,CoIP	Nat Commun; Oxidative stress-induced FABP5 S-glutathionylation protects against acute lung injury by suppressing inflammation in macrophages.; Authors - Yuxian Guo
mouse	WB	Biomaterials; MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.; Authors - Mingming Song
human	IF	Mol Ther; Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.; Authors - Junyi Hu
human	WB	EMBO Rep; Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.; Authors - Yang Zhang
rat	WB	Phytomedicine; Podophyllotoxin via SIRT1/PPAR /NF-κB axis induced cardiac injury in rats based on the toxicological evidence chain (TEC) concept; Authors - Tao Jiang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kenzo (Verified Customer) (01-09-2023)

Robust immunoreactivity detected.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse nerve

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