Anticorps Polyclonal de lapin anti-FABP7-Specific

FABP7-Specific Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 14836-1-AP

Synonymes

B FABP, BLBP, Brain lipid binding protein, FABP7, FABP7-Specific, FABPB, Fatty acid binding protein 7, MRG

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Galerie de données de validation

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  WB analysis of human brain using 14836-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14836-1-AP (FABP7-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 14836-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 14836-1-AP (FABP7-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 14836-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14836-1-AP (FABP7-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14836-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14836-1-AP (FABP7-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 14836-1-AP

  Immunofluorescent analysis of (-20°C Acetone) fixed rat brain tissue using FABP7-Specific antibody (14836-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of rat brain using 14836-1-AP

  Immunofluorescent analysis of (-20°C Acetone) fixed rat brain tissue using FABP7-Specific antibody (14836-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules U-251
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de rat,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

14836-1-AP cible FABP7-Specific dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: fatty acid binding protein 7, brain
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 14-15 kDa
• Numéro d’acquisition GenBank: NM_001446
• Symbole du gène: FABP7
• Identification du gène (NCBI): 2173
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FABP7 (Fatty Acid Binding Protein-7), also known as B-FABP (Brain Fatty Acid-Binding Protein), is a member of FABP family which are intracellular proteins involved in lipid metabolism. Normally FABP7 is mainly expressed in brain and other neural tissues and is important in early nervous system development. In addition, FABP7 is considered a marker for some brain tumors and may have a role in tumorigenesis in the peripheral nervous system and in several extra nervous system neoplasms such as breast cancer, renal cancer, and melanoma. This antibody specially recognizes FABP7 and not cross-react with other FABPs.

Publications

Species	Application	Title
	IF	Nucleic Acids Res; H2A.Z.1 crosstalk with H3K56-acetylation controls gliogenesis through the transcription of folate receptor.; Authors - Libo Su
human	IHC	Ther Adv Med Oncol; Development and external validation of a composite immune-clinical prognostic model associated with EGFR mutation in East-Asian patients with lung adenocarcinoma.; Authors - Chengming Liu