Anticorps Polyclonal de lapin anti-FADD

FADD Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14906-1-PBS

Synonymes

FAS-associated death domain protein, FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, MORT1

---

Galerie de données de validation

• 

  WB analysis using 14906-1-AP (same clone as 14906-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse pancreas using 14906-1-AP (same clone as 14906-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 14906-1-AP (same clone as 14906-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14906-1-AP (same clone as 14906-1-PBS)

  mouse bone marrow lysates were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

• 

  IP experiment of A549 using 14906-1-AP (same clone as 14906-1-PBS)

  IP result of anti-FADD (IP:14906-1-AP, 4ug; Detection:14906-1-AP 1:300) with A549 cells lysate 3040ug. This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 14906-1-AP (same clone as 14906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 14906-1-AP (same clone as 14906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 14906-1-AP (same clone as 14906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 14906-1-AP (same clone as 14906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 14906-1-AP (same clone as 14906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 14906-1-AP (same clone as 14906-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FADD antibody (14906-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

14906-1-PBS cible FADD dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FADD Protéine recombinante Ag6701
• Nom complet: Fas (TNFRSF6)-associated via death domain
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 23-30 kDa
• Numéro d’acquisition GenBank: BC000334
• Symbole du gène: FADD
• Identification du gène (NCBI): 8772
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Fas-Associated protein with Death Domain (FADD), also called MORT1 or GIG3, is encoded by the FADD gene. FADD is an adaptor protein that bridges members of the tumor necrosis factor receptor superfamily, such as the Fas-receptor, to procaspases 8 and 10 to form the death-inducing signaling complex (DISC) during apoptosis. As well as its most well known role in apoptosis, FADD has also been seen to play a role in other processes including proliferation, cell cycle regulation and development. FADD has a calculated molecular mass of 23 kDa and always can be detected as 23-30 kDa (PMID: 15390286, 22864571, 17977957)

{{ptg:RelatedPrimaryAntibodies}}