Anticorps Monoclonal anti-FADS2

FADS2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(7)

CloneNo.

1G4B10

N° de cat : 68026-1-Ig

Synonymes

EC:1.14.19.3, Delta-6 desaturase, D6D, Acyl-CoA 6-desaturase, 1G4B10

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Galerie de données de validation

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  WB analysis of PC-3 using 68026-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 68026-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 68026-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 68026-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 68026-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 68026-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 68026-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 68026-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 68026-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using FADS2 antibody (68026-1-Ig, Clone: 1G4B10 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules A549, cellules Jurkat, cellules LNCaP, cellules U2OS, tissu hépatique de rat
• Résultats positifs en IHC: tissu hépatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below

Informations sur le produit

68026-1-Ig cible FADS2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FADS2 Protéine recombinante Ag27715
• Nom complet: fatty acid desaturase 2
• Masse moléculaire calculée: 445 aa, 49 kDa
• Poids moléculaire observé: 42-45 kDa
• Numéro d’acquisition GenBank: BC009011
• Symbole du gène: FADS2
• Identification du gène (NCBI): 9415
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fatty acid desaturase 2 (FADS2) is responsible for the first desaturation reaction in the synthesis of highly unsaturated fatty acids (HUFAs), such as arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3), and is involved in Mead acid (20:3n-9) production during essential fatty acid deficiency (EFAD) (PMID: 29353041). This is important when temperatures changes and the membrane is under distress. It has 4 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	Proteomics; Proteomics coupled transcriptomics reveals lipopolysaccharide inhibiting peroxisome proliferator-activated receptors signalling pathway to reduce lipid droplets accumulation in mouse liver; Authors - Haiying Ran
human	WB	Sci Rep; FADS2 confers SCD1 inhibition resistance to cancer cells by modulating the ER stress response; Authors - Toshikatsu Ikeda ; KD Validated
human	WB	FASEB J; Lysosomal membrane protein TMEM106B modulates hematopoietic stem and progenitor cell proliferation and differentiation by regulating LAMP2A stability; Authors - Di Guo
mouse	WB	Prostaglandins Leukot Essent Fatty Acids; Fatty acid synthase global inducible knockout does not alter brain fatty acid concentrations but attenuates cholesterol synthesis in the adult mouse; Authors - Drew R Seeger
rat	WB	Lipids; Exogenous oxidized phytosterol may modulate linoleic acid metabolism through upregulation of fatty acid desaturase in rats; Authors - Tomonari Koyama
human	WB	Mol Biol Rep; FADS2 inhibits colorectal cancer cell proliferation by regulating ferroptosis through SLC7A11/GPX4; Authors - Qinghui Yang

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