Anticorps Monoclonal anti-FADS2

FADS2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G4B10

N° de cat : 68026-1-PBS

Synonymes

EC:1.14.19.3, Delta-6 desaturase, D6D, Acyl-CoA 6-desaturase, 1G4B10

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Galerie de données de validation

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  WB analysis of PC-3 using 68026-1-Ig (same clone as 68026-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68026-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 68026-1-Ig (same clone as 68026-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68026-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 68026-1-Ig (same clone as 68026-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68026-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 68026-1-Ig (same clone as 68026-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68026-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 68026-1-Ig (same clone as 68026-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68026-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 68026-1-Ig (same clone as 68026-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68026-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 68026-1-Ig (same clone as 68026-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68026-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 68026-1-Ig (same clone as 68026-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68026-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 68026-1-Ig (same clone as 68026-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using FADS2 antibody (68026-1-Ig, Clone: 1G4B10 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68026-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68026-1-PBS cible FADS2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FADS2 Protéine recombinante Ag27715
• Nom complet: fatty acid desaturase 2
• Masse moléculaire calculée: 445 aa, 49 kDa
• Poids moléculaire observé: 42-45 kDa
• Numéro d’acquisition GenBank: BC009011
• Symbole du gène: FADS2
• Identification du gène (NCBI): 9415
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Fatty acid desaturase 2 (FADS2) is responsible for the first desaturation reaction in the synthesis of highly unsaturated fatty acids (HUFAs), such as arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3), and is involved in Mead acid (20:3n-9) production during essential fatty acid deficiency (EFAD) (PMID: 29353041). This is important when temperatures changes and the membrane is under distress. It has 4 isoforms produced by alternative splicing.

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