Anticorps Polyclonal de lapin anti-FADS2

• Phare
• Validé par KD/KO

FADS2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(10)

Avis (2)

N° de cat : 28034-1-AP

Synonymes

EC:1.14.19.3, Delta-6 desaturase, D6D, Acyl-CoA 6-desaturase

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Galerie de données de validation

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  WB analysis using 28034-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 28034-1-AP (FADS2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 28034-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 28034-1-AP (FADS2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 28034-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 28034-1-AP (FADS2-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 28034-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28034-1-AP (FADS2 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 28034-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28034-1-AP (FADS2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules L02, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu cardiaque de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 2 publications below

Informations sur le produit

28034-1-AP cible FADS2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FADS2 Protéine recombinante Ag27709
• Nom complet: fatty acid desaturase 2
• Masse moléculaire calculée: 445 aa, 49 kDa
• Poids moléculaire observé: 46-52 kDa
• Numéro d’acquisition GenBank: BC009011
• Symbole du gène: FADS2
• Identification du gène (NCBI): 9415
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fatty acid desaturase 2 (FADS2) is responsible for the first desaturation reaction in the synthesis of highly unsaturated fatty acids (HUFAs), such as arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3), and is involved in Mead acid (20:3n-9) production during essential fatty acid deficiency (EFAD) (PMID: 29353041). This is important when temperatures changes and the membrane is under distress. It has 4 isoforms and can be detected as 46-52 kDa.

Publications

Species	Application	Title
human	WB	Cell Chem Biol; FADS2-dependent fatty acid desaturation dictates cellular sensitivity to ferroptosis and permissiveness for hepatitis C virus replication.; Authors - Daisuke Yamane ; KD Validated
human	WB	FASEB J; PMID: 31199678; Authors - Ge Liu
human,mouse	WB,IF	Immun Inflamm Dis; Identification of Ferroptosis-Related Gene in Age-Related Macular Degeneration Using Machine Learning; Authors - Meijiang Zhu
	IF	BME Front; Multimodal Metabolic Imaging Reveals Pigment Reduction and Lipid Accumulation in Metastatic Melanoma; Authors - Hyeon Jeong Lee
mouse	WB	FASEB J; Comparing Soy and Milk Protein Regulation of Hepatic Omega-3 Fatty Acid Biosynthesis; Authors - Melissa Gonzalez-Soto
mouse	WB	Pharmacol Res; Breaking the synergism of iron overload and miR-122 to rescue lipid accumulation and peroxidation in MASLD; Authors - Yuxiao Tang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xianfeng (Verified Customer) (02-18-2020)

Works well with overexpressed mouse FADS2 but no signal with endogenouse mouse FADS2.

• Applications: Western Blot,

FADS2 Antibody Western Blot, validation ( dilution) in (Cat no:28034-1-AP)

FH

XIANFENG (Verified Customer) (02-06-2020)

Works well with overexpressed mouse FADS2, but does not work with endogenous mouse FADS2 (mouse liver) .

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000

FADS2 Antibody Western Blot, validation (1:1000 dilution) in (Cat no:28034-1-AP)

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