Anticorps Recombinant de lapin anti-FADS2

FADS2 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241863D2

N° de cat : 84527-4-RR

Synonymes

241863D2, Acyl-CoA 6-desaturase, D6D, Delta-6 desaturase, EC:1.14.19.3

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Galerie de données de validation

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  WB analysis of L02 using 84527-4-RR

  L02 cells were subjected to SDS PAGE followed by western blot with 84527-4-RR (FADS2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84527-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84527-4-RR (FADS2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 84527-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84527-4-RR (FADS2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 84527-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84527-4-RR against Human FADS2-Specific were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules L02, cellules A549, cellules Jurkat, cellules LNCaP, cellules PC-3, tissu hépatique de rat, tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84527-4-RR cible FADS2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FADS2 Protéine recombinante Ag27715
• Nom complet: fatty acid desaturase 2
• Masse moléculaire calculée: 445 aa, 49 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC009011
• Symbole du gène: FADS2
• Identification du gène (NCBI): 9415
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fatty acid desaturase 2 (FADS2) is responsible for the first desaturation reaction in the synthesis of highly unsaturated fatty acids (HUFAs), such as arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3), and is involved in Mead acid (20:3n-9) production during essential fatty acid deficiency (EFAD) (PMID: 29353041). This is important when temperatures changes and the membrane is under distress. It has 4 isoforms produced by alternative splicing.

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