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Anticorps Recombinant de lapin anti-FADS2

FADS2 Recombinant Antibody for IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241863G3

N° de cat : 84527-5-RR

Synonymes

241863G3, Acyl-CoA 6-desaturase, D6D, Delta-6 desaturase, EC:1.14.19.3

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FADS2-Specific Recombinant antibody (84527-5-RR)

    IF Staining of HepG2 using 84527-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FADS2-Specific antibody (84527-5-RR, Clone: 241863G3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84527-5-RR

    Affinity and Kinetic Characterization of 84527-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84527-5-RR against Human FADS2-Specific were performed. The affinity constant is below 1 pM.

Applications testées

Résultats positifs en IF/ICCcellules HepG2,

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

84527-5-RR cible FADS2 dans les applications de IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène FADS2 Protéine recombinante Ag27715
Nom complet fatty acid desaturase 2
Masse moléculaire calculée 445 aa, 49 kDa
Numéro d’acquisition GenBankBC009011
Symbole du gène FADS2
Identification du gène (NCBI) 9415
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Protein A purfication
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fatty acid desaturase 2 (FADS2) is responsible for the first desaturation reaction in the synthesis of highly unsaturated fatty acids (HUFAs), such as arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3), and is involved in Mead acid (20:3n-9) production during essential fatty acid deficiency (EFAD) (PMID: 29353041). This is important when temperatures changes and the membrane is under distress. It has 4 isoforms and can be detected as 46-52 kDa.

Protocole

Product Specific Protocols
IF protocol for FADS2 antibody 84527-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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