Anticorps Polyclonal de lapin anti-FAF2

• Phare
• Validé par KD/KO

FAF2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16251-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 16251-1-AP (same clone as 16251-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16251-1-AP (FAF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

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  WB analysis using 16251-1-AP (same clone as 16251-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16251-1-AP (FAF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 16251-1-AP (same clone as 16251-1-PBS)

  WB result of FAF2 antibody (16251-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAF2 transfected HeLa cells. This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 16251-1-AP (same clone as 16251-1-PBS)

  IP result of anti-FAF2 (IP:16251-1-AP, 3ug; Detection:16251-1-AP 1:1000) with mouse brain tissue lysate 5000ug. This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 16251-1-AP (same clone as 16251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16251-1-AP (FAF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 16251-1-AP (same clone as 16251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16251-1-AP (FAF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 16251-1-AP (same clone as 16251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16251-1-AP (FAF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 16251-1-AP (same clone as 16251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16251-1-AP (FAF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16251-1-PBS cible FAF2 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FAF2 Protéine recombinante Ag9236
• Nom complet: Fas associated factor family member 2
• Masse moléculaire calculée: 445 aa, 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC014001
• Symbole du gène: FAF2
• Identification du gène (NCBI): 23197
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Protein ETEA (FAF2, or UBXD8) is a homolog to Fas-associated factor 1 (FAF1), which is involved in Fas-mediated apoptosis. ETEA protein directly interacts with and negatively regulates neurofibromin. ETEA contains both UBA and UBX domains and overexpression of ETEA downregulates neurofibromin in human cells. It may play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. ETEA is highly expressed in peripheral blood of patients with atopic dermatitis (AD) compared to normal individuals, and may regulate the resistance to apoptosis that is observed in T cells and eosinophils of AD patients.

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