Anticorps Monoclonal anti-FAH

FAH Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2F8E11

N° de cat : 67986-1-PBS

Synonymes

2F8E11, Beta-diketonase, EC:3.7.1.2, FAA, Fumarylacetoacetate hydrolase

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Galerie de données de validation

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  WB analysis using 67986-1-Ig (same clone as 67986-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67986-1-Ig (FAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67986-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 67986-1-Ig (same clone as 67986-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67986-1-Ig (FAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67986-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 67986-1-Ig (same clone as 67986-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67986-1-Ig (FAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67986-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 67986-1-Ig (same clone as 67986-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67986-1-Ig (FAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67986-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67986-1-Ig (same clone as 67986-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using FAH antibody (67986-1-Ig, Clone: 2F8E11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67986-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67986-1-Ig (same clone as 67986-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAH antibody (67986-1-Ig, Clone: 2F8E11 ) at dilution of 1:150 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67986-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67986-1-PBS cible FAH dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FAH Protéine recombinante Ag6704
• Nom complet: fumarylacetoacetate hydrolase (fumarylacetoacetase)
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC002527
• Symbole du gène: FAH
• Identification du gène (NCBI): 2184
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FAH is also named as FAA(fumarylacetoacetase), the last enzyme in tyrosine degradation, functionally important in the liver and kidney(PMID:8473520). FAH is a metabolic enzyme catalyzing the last step of tyrosine and phenylalanine catabolism: the hydrolysis of fumarylacetoacetate into acetoacetate and fumarate. In humans, deficiency of this activity is associated with the metabolic disease hereditary tyrosinaemia type I, which is also known as hepatorenal tyrosinaemia(PMID:17768357).

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