Anticorps Recombinant de lapin anti-FAK

FAK Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242295E4

N° de cat : 81334-5-PBS

Synonymes

PTK2, EC:2.7.10.2, FADK 1, FAK1, Focal adhesion kinase 1

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Galerie de données de validation

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  WB analysis using 81334-5-RR (same clone as 81334-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81334-5-RR (FAK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81334-5-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 81334-5-RR (same clone as 81334-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using FAK antibody (81334-5-RR, Clone: 242295E4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81334-5-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 81334-5-RR (same clone as 81334-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using FAK antibody (81334-5-RR, Clone: 242295E4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81334-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81334-5-RR

  Biolayer interferometry (BLl) kinetic assays of 81334-5-RR against Human FAK were performed. The affinity constant is 2.03 nM.

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Informations sur le produit

81334-5-PBS cible FAK dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FAK Protéine recombinante Ag3331
• Nom complet: PTK2 protein tyrosine kinase 2
• Masse moléculaire calculée: 1052 aa, 119 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC028733
• Symbole du gène: FAK
• Identification du gène (NCBI): 5747
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971).

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