Anticorps Recombinant de lapin anti-Phospho-FAK (Tyr397)

Phospho-FAK (Tyr397) Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

240630F8

Avis (1)

N° de cat : 83933-1-RR

Synonymes

FAK (Tyr397), p FAK, p FAK (Tyr397), p FAK Tyr397, p-FAK

---

Galerie de données de validation

• 

  WB analysis using 83933-1-RR

  Non-treated and pervanadate treated HeLa cells were subjected to SDS PAGE followed by western blot with 83933-1-RR (Phospho-FAK (Tyr397) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

• 

  IF Staining of A549 using 83933-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Phospho-FAK (Tyr397) antibody (83933-1-RR, Clone: 240630F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of A549 using 83933-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Phospho-FAK (Tyr397) antibody (83933-1-RR, Clone: 240630F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

---

Applications testées

• Résultats positifs en WB: pervanadate treated HeLa cells,
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

83933-1-RR cible Phospho-FAK (Tyr397) dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: PTK2 protein tyrosine kinase 2
• Masse moléculaire calculée: 1052 aa, 119 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC028733
• Symbole du gène: FAK
• Identification du gène (NCBI): 5747
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative splicing and contains several phosphorylation and SUMOylation modification sites.

Publications

Species	Application	Title
human	WB	Eur J Med Chem; Discovery of a potent anticancer agent against pancreatic ductal adenocarcinoma targeting FAK with DFG-out state and JAK/Aurora kinases; Authors - Rong-Hong Zhang
human	WB	IUBMB Life; Tinagl1 restores tamoxifen sensitivity and blocks fibronectin-induced EMT by simultaneously blocking the EGFR and β1-integrin/FAK signaling pathways in tamoxifen-resistant breast cancer cells; Authors - Jie Yuan
human	WB,IF	iScience; WTX-L/β-arrestin2/LCN2 axis controls vulnerability to ferroptosis in gastric cancer; Authors - Yangwei Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Udesh (Verified Customer) (08-12-2025)

Worked well for both IF and WB

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: WB 1:1000, IF, 1:300
• Cell Tissue Type: MSCs

{{ptg:RelatedPrimaryAntibodies}}