Anticorps Monoclonal anti-FAM114A1

FAM114A1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1B11D6

N° de cat : 67926-1-Ig

Synonymes

FAM114A1, Noxp20, Protein FAM114A1, Protein NOXP20

---

Galerie de données de validation

• 

  WB analysis using 67926-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67926-1-Ig (FAM114A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67926-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67926-1-Ig (FAM114A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 67926-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 67926-1-Ig (FAM114A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 67926-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67926-1-Ig (FAM114A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 67926-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67926-1-Ig (FAM114A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 67926-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAM114A1 antibody (67926-1-Ig, Clone: 1B11D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules U2OS, cellules A549, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules LNCaP, cellules NIH/3T3, cellules PC-12
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

67926-1-Ig cible FAM114A1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FAM114A1 Protéine recombinante Ag17289
• Nom complet: family with sequence similarity 114, member A1
• Masse moléculaire calculée: 563 aa, 61 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC040452
• Symbole du gène: FAM114A1
• Identification du gène (NCBI): 92689
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FAM114A1, also named as Nervous system overexpressed protein 20, is a 563 amino acid protein, which belongs to the FAM114 family. FAM114A1 localizes in the cytoplasm and may play a role in neuronal cell development. The calculated molecular weight of FAM114A1 is 61 kDa, but the modified FAM114A1 may be 70-80 kDa protein.

Publications

Species	Application	Title
human	IHC	Front Oncol; RAB42 Promotes Glioma Pathogenesis via the VEGF Signaling Pathway.; Authors - Baoling Liu

{{ptg:RelatedPrimaryAntibodies}}