Anticorps Polyclonal de lapin anti-FAM127

FAM127 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20282-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of PC-3 using 20282-1-AP (same clone as 20282-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20282-1-AP (FAM127B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 20282-1-AP (same clone as 20282-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20282-1-AP (FAM127B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

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  IP experiment of PC-3 using 20282-1-AP (same clone as 20282-1-PBS)

  IP result of anti-FAM127 (IP:20282-1-AP, 4ug; Detection:20282-1-AP 1:500) with PC-3 cells lysate 3960 ug. This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 20282-1-AP (same clone as 20282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 20282-1-AP (FAM127B antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 20282-1-AP (same clone as 20282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 20282-1-AP (FAM127B antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 20282-1-AP (same clone as 20282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 20282-1-AP (FAM127B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 20282-1-AP (same clone as 20282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 20282-1-AP (FAM127B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 20282-1-AP (same clone as 20282-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using FAM127B antibody 20282-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20282-1-PBS cible FAM127 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FAM127 Protéine recombinante Ag13930
• Nom complet: family with sequence similarity 127, member B
• Masse moléculaire calculée: 113 aa, 13 kDa
• Poids moléculaire observé: 13 kDa
• Numéro d’acquisition GenBank: BC000393
• Symbole du gène: FAM127B
• Identification du gène (NCBI): 26071
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The FAM127 family currently has three members, namely FAM127A (also called RTL8C and CXX1), FAM127B (also called CXX1B and RTL8A), and FAM127C (also called RTL8B and CXX1C). They are located on chromosomes and their functions are unknown. All three are composed of 113 amino acids and have extremely high sequence similarity. The immunogen of this antibody has a sequence similarity of more than 95% with members of the FAM127 family. Theoretically, the antibody can recognize all members of the FAM127 family.

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