Anticorps Polyclonal de lapin anti-FAM127

FAM127 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 20282-1-AP

Synonymes

CXX1, CXX1b, CXX1C, FAM127, FAM127 A/B/C, MAR8, MAR8A, MAR8B, MAR8C, RTL8, RTL8 A/B/C, RTL8A, RTL8B, RTL8C

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Galerie de données de validation

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  WB analysis of PC-3 using 20282-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20282-1-AP (FAM127B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20282-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20282-1-AP (FAM127B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 20282-1-AP

  IP result of anti-FAM127B (IP:20282-1-AP, 4ug; Detection:20282-1-AP 1:500) with PC-3 cells lysate 3960 ug.

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  IHC staining of human colon using 20282-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20282-1-AP (FAM127B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 20282-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20282-1-AP (FAM127B antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 20282-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20282-1-AP (FAM127B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 20282-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20282-1-AP (FAM127B antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 20282-1-AP

  Immunofluorescent analysis of HepG2 cells, using FAM127B antibody 20282-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules HeLa
• Résultats positifs en IP: cellules PC-3,
• Résultats positifs en IHC: tissu de côlon humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

20282-1-AP cible FAM127 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FAM127 Protéine recombinante Ag13930
• Nom complet: family with sequence similarity 127, member B
• Masse moléculaire calculée: 113 aa, 13 kDa
• Poids moléculaire observé: 13 kDa
• Numéro d’acquisition GenBank: BC000393
• Symbole du gène: FAM127B
• Identification du gène (NCBI): 26071
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The FAM127 family currently has three members, namely FAM127A (also called RTL8C and CXX1), FAM127B (also called CXX1B and RTL8A), and FAM127C (also called RTL8B and CXX1C). They are located on chromosomes and their functions are unknown. All three are composed of 113 amino acids and have extremely high sequence similarity. The immunogen of this antibody has a sequence similarity of more than 95% with members of the FAM127 family. Theoretically, the antibody can recognize all members of the FAM127 family.

Publications

Species	Application	Title
human,mouse	WB	Cell Mol Life Sci; RTL8 promotes nuclear localization of UBQLN2 to subnuclear compartments associated with protein quality control.; Authors - Harihar Milaganur Mohan
	IP	J Biol Chem; Global proteomics of Ubqln2-based murine models of ALS.; Authors - Alexandra M Whiteley

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Harihar (Verified Customer) (01-28-2024)

The antibody works well for Western blots and we have validated it in knockout cell lines. There is however a lot of high molecular weight background bands. It is not suitable for IF.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500 (WB)
• Cell Tissue Type: HEK293, HeLa, Kolf 2 IPSCs

FAM127 Antibody Western Blot validation (1:500 (WB) dilution) in HEK293, HeLa, Kolf 2 IPSCs (Cat no:20282-1-AP)