Anticorps Polyclonal de lapin anti-FAM162A

FAM162A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 32034-1-PBS

Synonymes

C3orf28, DC16, E2IG5, E2-induced gene 5 protein, FWP001

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Galerie de données de validation

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  WB analysis using 32034-1-AP (same clone as 32034-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 32034-1-AP (FAM162A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32034-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 32034-1-AP (same clone as 32034-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 32034-1-AP (FAM162A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32034-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 32034-1-AP (same clone as 32034-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 32034-1-AP (FAM162A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32034-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 32034-1-AP (same clone as 32034-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using FAM162A antibody (32034-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 32034-1-PBS in a different storage buffer formulation.

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Informations sur le produit

32034-1-PBS cible FAM162A dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FAM162A Protéine recombinante Ag36569
• Nom complet: family with sequence similarity 162, member A
• Masse moléculaire calculée: 154 aa, 17 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC015060
• Symbole du gène: FAM162A
• Identification du gène (NCBI): 26355
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Family with sequence similarity 162 member A (FAM162A, also known as E2IG5, HGTD-P, and C3orf28) is a mitochondrial apoptotic protein, which is known as an HIF-1 alpha-responsive pro-apoptotic molecule (PMID: 15082785; 16698020; 22776087). When FAM162A was overexpressed, the hypoxia signal was sent directly to the mitochondria and caused cell death (PMID: 16698020).

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