Anticorps Polyclonal de lapin anti-FAM175A

FAM175A Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 14366-1-PBS

Synonymes

UNQ496/PRO1013, Coiled-coil domain-containing protein 98, CCDC98, BRCA1-A complex subunit Abraxas 1, ABRAXAS1

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Galerie de données de validation

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  WB analysis using 14366-1-AP (same clone as 14366-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 14366-1-AP (FAM175A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14366-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 14366-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14366-1-AP (FAM175A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 14366-1-AP (same clone as 14366-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 14366-1-AP (FAM175A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14366-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 14366-1-AP (same clone as 14366-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 14366-1-AP (FAM175A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14366-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 14366-1-AP (same clone as 14366-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 14366-1-AP (FAM175A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14366-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14366-1-AP (same clone as 14366-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FAM175A antibody (14366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14366-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14366-1-PBS cible FAM175A dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FAM175A Protéine recombinante Ag5688
• Nom complet: family with sequence similarity 175, member A
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC039573
• Symbole du gène: FAM175A
• Identification du gène (NCBI): 84142
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The family with sequence similarity 175 member A gene (FAM175A; also known as ABRAXAS1, CCDC98 and ABRA1), a member of the DNA repair family, contributes to the BRCA1 (BRCA1 DNA repair associated)-dependent DNA damage response and is associated with age at natural menopause.

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