Anticorps Polyclonal de lapin anti-FAM175B

FAM175B Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 26868-1-AP

Synonymes

Abraxas brother protein 1, ABRAXAS2, ABRO1, BRISC complex subunit Abraxas 2, BRISC complex subunit Abro1

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Galerie de données de validation

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  WB analysis of HL-60 using 26868-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 26868-1-AP (FAM175B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 26868-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26868-1-AP (FAM175B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 26868-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26868-1-AP (FAM175B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 26868-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26868-1-AP (FAM175B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 26868-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26868-1-AP (FAM175B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HEK-293, tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

26868-1-AP cible FAM175B dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FAM175B Protéine recombinante Ag22121
• Nom complet: family with sequence similarity 175, member B
• Masse moléculaire calculée: 415 aa, 47 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC008999
• Symbole du gène: FAM175B
• Identification du gène (NCBI): 23172
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FAM175B (also known as ABRO1/KIAA0157) is a scaffold subunit of the BRCC36-containing deubiquitinase complex (BRISC) that specifically cleaves Lys63-linked polyubiquitin chains, thereby modulating DNA-damage responses and receptor trafficking. Originally identified as a binding partner of JunD and ATF4, FAM175B stabilizes ATF4 by inhibiting its ubiquitin-dependent degradation, leading to activation of the ATF4-CHOP pathway and induction of apoptosis under endoplasmic reticulum stress. In esophageal squamous cell carcinoma, FAM175B expression is down-regulated and acts as a p53-independent tumor suppressor; restoring its expression reduces proliferation and increases apoptosis of p53-mutated tumor cells. Collectively, FAM175B serves as a critical scaffold that links deubiquitinase activity to transcriptional and pro-apoptotic programs, offering a potential biomarker for early diagnosis and therapeutic targeting in cancers with p53 dysfunction (PMID: 30854784).

Publications

Species	Application	Title
human	WB	Autophagy; A bacterial RING ubiquitin ligase triggering stepwise degradation of BRISC via TOLLIP-mediated selective autophagy manipulates host inflammatory response; Authors - Xinming Pan

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