• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-Piezo1 (extracellular domain)

Piezo1 (extracellular domain) Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (5)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(199)

N° de cat : 15939-1-AP

Synonymes

FAM38A, PIEZO1, KIAA0233, Membrane protein induced by beta-amyloid treatment, Piezo-type mechanosensitive ion channel component 1



Applications testées

Résultats positifs en WBcellules HeLa, cellules hTERT-RPE1, cellules SGC-7901
Résultats positifs en IHCtissu cutané humain, tissu de cancer de la peau humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules THP-1,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

15939-1-AP cible Piezo1 (extracellular domain) dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéerat, canin, Humain, poisson-zèbre, porc, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Piezo1 (extracellular domain) Protéine recombinante Ag7791
Nom complet family with sequence similarity 38, member A
Masse moléculaire calculée 286 kDa
Poids moléculaire observé 233-286 kDa
Numéro d’acquisition GenBankBC008073
Symbole du gène Piezo1/FAM38A
Identification du gène (NCBI) 9780
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells that influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as Piezo1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Piezo1 also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in the gene encoding Piezo1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1. (PMID: 20016066, 28416610)

Protocole

Product Specific Protocols
WB protocol for Piezo1 (extracellular domain) antibody 15939-1-APDownload protocol
IHC protocol for Piezo1 (extracellular domain) antibody 15939-1-APDownload protocol
IF protocol for Piezo1 (extracellular domain) antibody 15939-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
zebrafishIF

Nature

Skin cells undergo asynthetic fission to expand body surfaces in zebrafish.

Authors - Keat Ying Chan
  • KO Validated
humanWB

Nature

Piezo1 integration of vascular architecture with physiological force.

Authors - Jing Li
  • KD Validated
zebrafish,mouseWB

Nature

Crowding induces live cell extrusion to maintain homeostatic cell numbers in epithelia.

Authors - Eisenhoffer George T GT
  • KD Validated
zebrafish,canineWB,IF

Nature

Mechanical stretch triggers rapid epithelial cell division through Piezo1.

Authors - S A Gudipaty
  • KO Validated
mouseIHC

Cell

RNA Sensing by Gut Piezo1 Is Essential for Systemic Serotonin Synthesis.

Authors - Erika Sugisawa
humanWB

Blood

Mechanosensation by endothelial PIEZO1 is required for leukocyte diapedesis.

Authors - ShengPeng Wang
  • KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Yao (Verified Customer) (09-24-2025)

I used this antibody for the kidney slides of wild-type mice, it showed positive staining that matches the expected pattern

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Kidney
Piezo1 (extracellular domain) Antibody Immunohistochemistry validation (1:100 dilution) in Kidney (Cat no:15939-1-AP)
FH

Hyun (Verified Customer) (07-23-2025)

This antibody works well for western blot.

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: rat brain tissue
FH

Jason (Verified Customer) (02-11-2025)

The antibody works well. However, due to the large protein size, obtaining sharp and clean Western blot bands is challenging for me. In fluorescence staining, the knockout cells were stained similarly to the control cells, suggesting non-specific staining in IF.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:500 for WB; 1:200 for IF
  • Cell Tissue Type: Human Dermal Microvascular Endothelial Cell
Piezo1 (extracellular domain) Antibody Western Blot,Immunofluorescence validation (1:500 for WB; 1:200 for IF dilution) in Human Dermal Microvascular Endothelial Cell (Cat no:15939-1-AP)
FH

Hyojin (Verified Customer) (01-19-2024)

I like the quality of antibody. Thank you for generating.

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:200 for IF 1:1000 for WB
  • Cell Tissue Type: mouse/human
FH

Sammy (Verified Customer) (12-04-2023)

Ab detects a band at the correct size but the signal is weak. This works only when detected via HRP/Chemi. NIR-conjugated antibodies do not detect this band.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 in 1000
  • Cell Tissue Type: HUVEC
Piezo1 (extracellular domain) Antibody Western Blot validation (1 in 1000 dilution) in HUVEC (Cat no:15939-1-AP)
FH

Xiaonan (Verified Customer) (02-01-2023)

We did not see the band for Piezol.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse kidney
Piezo1 (extracellular domain) Antibody Western Blot validation (1:1000 dilution) in mouse kidney (Cat no:15939-1-AP)
FH

LUNFENG (Verified Customer) (01-27-2020)

GOOD

  • Applications: Immunofluorescence,
  • Cell Tissue Type: HEART
FH

Jie (Verified Customer) (01-23-2020)

Works with one band. Due to the large protein size, band does not always show sharp image.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hela cells
FH

Canzhao (Verified Customer) (01-17-2020)

It worked in western blot and showed nice band.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse heart tissue
FH

Zeinab (Verified Customer) (09-27-2019)

Worked great, Clean and Specific

  • Applications: Western Blot, Immunofluorescence,
  • Cell Tissue Type: Human Cardiac Microvascular Endothelial cells
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