Anticorps Recombinant de lapin anti-Piezo1 (extracellular domain)

Piezo1 (extracellular domain) Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

4B15

N° de cat : 82625-4-RR

Synonymes

FAM38A, PIEZO1, 4B15, KIAA0233, Membrane protein induced by beta-amyloid treatment

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Galerie de données de validation

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  WB analysis using 82625-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82625-4-RR (Piezo1 (extracellular domain) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82625-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82625-4-RR (Ag7791 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat dorsal root ganglion using 82625-4-RR

  Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 82625-4-RR (Piezo1 (extracellular domain) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of THP-1 using 82625-4-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using Piezo1 (extracellular domain) antibody (82625-4-RR, Clone: 4B15 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 82625-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82625-4-RR against Human Piezo1 (extracellular domain) were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules hTERT-RPE1, cellules SGC-7901
• Résultats positifs en IHC: rat dorsal root ganglion tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules THP-1,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below

Informations sur le produit

82625-4-RR cible Piezo1 (extracellular domain) dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Piezo1 (extracellular domain) Protéine recombinante Ag7791
• Nom complet: family with sequence similarity 38, member A
• Masse moléculaire calculée: 286 kDa
• Poids moléculaire observé: 233-286 kDa
• Numéro d’acquisition GenBank: BC008073
• Symbole du gène: Piezo1/FAM38A
• Identification du gène (NCBI): 9780
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells that influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as Piezo1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Piezo1 also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in the gene encoding Piezo1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1. (PMID: 20016066, 28416610)

Publications

Species	Application	Title
human	IHC	Commun Biol; Piezo1 promotes the progression of necrotizing enterocolitis by activating the Ca2(+)/CaMKII-dependent pathway; Authors - Zhenyu Li
human	IHC	bioRxiv; PIEZO1 Drives Trophoblast Fusion and Placental Development; Authors - Yang Zhang

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