Anticorps Monoclonal anti-FAM3C

FAM3C Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E10D5

N° de cat : 60282-1-PBS

Synonymes

2E10D5, GS3786, ILEI, Interleukin like EMT inducer, Interleukin-like EMT inducer

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Galerie de données de validation

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  WB analysis of fetal human brain using 60282-1-Ig (same clone as 60282-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60282-1-Ig (FAM3C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60282-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 60282-1-Ig (same clone as 60282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60282-1-Ig (FAM3C Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60282-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 60282-1-Ig (same clone as 60282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60282-1-Ig (FAM3C Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60282-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60282-1-Ig (same clone as 60282-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAM3C antibody (60282-1-Ig, Clone: 2E10D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60282-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60282-1-PBS cible FAM3C dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FAM3C Protéine recombinante Ag5516
• Nom complet: family with sequence similarity 3, member C
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC046932
• Symbole du gène: FAM3C
• Identification du gène (NCBI): 10447
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FAM3C, also known as ILEI, is a secreted protein belonging to the cytokine-like protein family. FAM3C is a signaling cytokine with a GG domain, hydrophobic leader sequence and an N-terminal signal peptide. A change in expression of this protein has been noted in pancreatic cancer-derived cells. FAM3C has been involved in the epithelial to mesenchymal transition, and retinal laminar formation processes in vertebrates. Also, FAM3C may play an important role in inner ear development and thus be involved in cell differentiation and proliferation during inner ear embryogenesis.

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