Anticorps Polyclonal de lapin anti-FAM57B

• Phare
• Validé par KD/KO

FAM57B Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (1)

N° de cat : 20760-1-AP

Synonymes

FAM57B, FP1188, Protein FAM57B

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Galerie de données de validation

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  WB analysis using 20760-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20760-1-AP (FAM57B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 20760-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 20760-1-AP (FAM57B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20760-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20760-1-AP (FAM57B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20760-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20760-1-AP (FAM57B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20760-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20760-1-AP (FAM57B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20760-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20760-1-AP (FAM57B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, tissu cérébral de rat, tissu de côlon de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

20760-1-AP cible FAM57B dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FAM57B Protéine recombinante Ag14707
• Nom complet: family with sequence similarity 57, member B
• Masse moléculaire calculée: 274 aa, 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC007892
• Symbole du gène: FAM57B
• Identification du gène (NCBI): 83723
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	iScience; 16pdel lipid changes in iPSC-derived neurons and function of FAM57B in lipid metabolism and synaptogenesis; Authors - Danielle L ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Danielle (Verified Customer) (12-16-2018)

This is the only antibody I've found that resolves FAM57B at the correct size. However, the antibody is not very clean with the SH-SY5Y cells and will show multiple bands.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: SH-SY5Y

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