Anticorps Polyclonal de lapin anti-FAM72

• Phare
• Validé par KD/KO

FAM72 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 21203-1-AP

Synonymes

FAM72A, UGENE, Protein FAM72A, LMPIP, LMP1-induced protein

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Galerie de données de validation

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  WB analysis using 21203-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21203-1-AP (FAM72 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21203-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21203-1-AP (FAM72 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 21203-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21203-1-AP (FAM72A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 21203-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21203-1-AP (FAM72 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 21203-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21203-1-AP (FAM72 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules HeLa, cellules Raji, tissu splénique de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

21203-1-AP cible FAM72 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FAM72 Protéine recombinante Ag15566
• Nom complet: family with sequence similarity 72, member A
• Masse moléculaire calculée: 149 aa, 17 kDa
• Poids moléculaire observé: 15-20 kDa
• Numéro d’acquisition GenBank: BC035696
• Symbole du gène: FAM72
• Identification du gène (NCBI): 729533
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FAM72A, also named as LMIP and UGENE, is a mitochondrial protein which regulates cellular reactive oxygen species metabolism. It's up-regulated in malignant colon cancers, compared to normal colon and colon adenomas. FAM72 has some members A/B/C/D with higher homology. This antibody detects all the members of FAM72.

Publications

Species	Application	Title
human,mouse	IHC,WB	Front Oncol; Integrated systemic analysis of FAM72A to identify its clinical relevance, biological function, and relationship to drug sensitivity in hepatocellular carcinoma; Authors - Qi Zhou ; KD Validated
human	WB	Int J Mol Sci; Prognostic and Immunological Implications of FAM72A in Pan-Cancer and Functional Validations; Authors - Yuwen Bai ; KD Validated

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