Anticorps Polyclonal de lapin anti-FAM76B

FAM76B Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 32882-1-AP

Synonymes

Protein FAM76B

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Galerie de données de validation

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  WB analysis using 32882-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32882-1-AP (FAM76B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 32882-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32882-1-AP (FAM76B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 32882-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32882-1-AP (FAM76B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: J774A.1 cells, cellules RAW 264.7, cellules SH-SY5Y, cellules U-937

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

32882-1-AP cible FAM76B dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FAM76B Protéine recombinante Ag36658
• Nom complet: family with sequence similarity 76, member B
• Masse moléculaire calculée: 39kDa，339aa
• Poids moléculaire observé: 36-39 kDa
• Numéro d’acquisition GenBank: NM_144664.4
• Symbole du gène: FAM76B
• Identification du gène (NCBI): 143684
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FAM76B is a nuclear protein with a molecular weight of about 39 kDa. Its amino acid sequence is highly conserved, and it is highly similar to FAM76B sequence of rats, mice and zebrafish. The protein contains histidine repeat sequence (poly-His domain) and ubiquitination site of Lys-225. FAM76B inhibits the inflammatory reaction mediated by NF-κB by affecting the cytoplasmic translocation of hnRNPA2B1. The expression of inflammatory factors (such as IL-6) increased significantly in FAM76B gene knockout mice, indicating that FAM76B has anti-inflammatory effect.

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